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ChomiX®-Magbeads Azide Coated Magnetic Beads
Cat. No.
CMXR-000003
CMXR-000004
Product Description
ChomiX®-Magbeads Azide Coated Magnetic Bead, non-cleavable linker group
ChomiX®-Magbeads Azide Coated Magnetic Beads, cleavable linker group
Product Introduction
ChomiX®-Magbeads Azide Coated Magnetic Beads are designed by covalently attaching an azide bioorthogonal group to the surface of the magnetic beads. Through non-cleavable ligation group (Figure A) and cleavable ligation group (Figure B)respectively, these beads can be applied to the enrichment of alkyne-modified macromolecules, including proteins, nucleic acids, antibodies and sugars. Over streptavidin (SA) coated magnetic beads, ChomiX®-Magbeads Azide Coated Magnetic Beads have the following advantages:
1. Through the efficient "click chemistry" reaction, the alkyne-modified macromolecules can be directly covalently bound to the magnetic beads, unlike the non-covalent and reversible interaction between streptavidin and biotin. Therefore, the product has higher recovery rate and better repeatability.
2. As there is no protein on the surface of the magnetic beads, the background peptide contamination due to enzymatic digestion will not be introduced. Furthermore, these beads reduce the non-specific adsorption to make samples cleaner, helping to improve the identification efficiency of low-abundance proteins.
3. Chemical molecular modified magnetic beads are stable, making them easier to transport and store.
4. Cleavable azide magnetic beads enable the mild release (using formic acid) of the peptides modified by the chemical probe, which is beneficial for the subsequent mass spectrometry identification.
5. The product is cost-effective, and improves experimental efficiency since it avoids streptavidin-biotin enrichment time.
Graph A: Azide Coated Magnetic Bead, non-cleavable linker group
Graph B: Azide Coated Magnetic Bead, cleavable group
Therefore, the development of ChomiX®-Magbeads azido-magnetic beads can greatly improve the efficiency of enrichment and separation of bioorthogonally tagged alkyne-modified macromolecules, thereby simplifying the experimental flow. In particular, the introduction of cleavable linker groups, which can efficiently release the target protein or peptides, facilitates protein quantification or identification of chemically probe-modified sites. Related study has been published in Journal of Proteome Research (J Proteome Res. 2023,3360-3367), an authoritative journal in the field of proteomics. The beads have a concentration of 10 mg/ml and a particle size of 1.0 μm, with a high azide loading capacity (30-50nmol/1mg) and exceptional hydrophilicity. Additionally, they possess superparamagnetic properties ensuring rapid magnetic response. Their excellent redispersion stability and magnetic stability effectively guarantee high protein binding rate, low non-specific adsorption, reaction uniformity and detection consistency.
Literature Case
In 2023, Professor Wang Chufrom Peking University, the chief scientist of ChomiX, published an efficient and concise chemical proteomics experiment scheme on Journal of Proteome Research with his group. This scheme uses cleavable azide magnetic beads to significantly shorten the analysis time for cysteine peptide group labelled with chemical probe (figure 1). Additionally, Figure 2 obviously exhibits a great improvement in the identification rate of modified peptides. Compared with the conventional streptavidin magnetic beads, azide magnetic beads show superior performance in terms of cost, time, and efficiency, having become a powerful tool in protein research, especially in chemical proteomics.
Figure 1:, Combined with azide coated magnetic beads with cleavable group, superTOP-ABPP experimental time can be reduced to 9 hours
Figure 2: When combined with azide coated magnetic beads with cleavable group, more than 10,000 cysteine peptides can be identified under the same conditions
Note
1. Before the first use, fully vortex and mix well to prevent changing the magnetic bead concentration. Avoid prolonged ultrasound to prevent damage to the surface of magnetic beads.
2. The bead storage solution contains 20% ethanol. Prior to use, perform magnetic separation and washing 2-3 times with pure water or buffer solution. Use the beads as soon as possible to avoid long storage;
3. Store at 4℃, and avoid freeze- thaw during the use.
4. Cleavable Azide magnetic beads should be kept away from acidic substances during storage and use.
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