Lysine Post-Translational Modifications

Among the 20 amino acid residues in proteins, lysine (K) is one of the most common targets for various post-translational modifications (PTMs). With the rapid development of high-resolution mass spectrometry (MS) and immunoprecipitation purification techniques, multiple acylation reactions on lysine have been discovered in the past decade, including acetylation, succinylation, malonylation, glutarylation, crotonylation, butyrylation, and lactylation. Reversible lysine post-translational modifications (Lys-PTMs) are ubiquitous biochemical processes that play indispensable roles in regulating numerous key cellular functions. Due to their close association with gene transcription, cellular metabolism, aging, and various diseases, lysine modifications have been a hot and focal point of scientific research.

Chomix has rich experience in identifying malonylation, for instance. Using the MalAM-yne probe for metabolic labeling at the cellular level, followed by click chemistry reactions at the lysate level, magnetic bead enrichment, and enzymatic digestion, specific identification of proteins with malonylation modifications is achieved through high-resolution mass spectrometry.

Chomix possesses advanced mass spectrometry technology capable of directly and accurately resolving various types of protein post-translational modifications and their specific sites. By cleverly combining separation enrichment techniques and isotope labeling, large-scale, high-throughput qualitative, and quantitative analyses of various modifications can be achieved, providing powerful technical support for in-depth research into protein post-translational modifications.

Our Advantages

1. Professional Expertise: With extensive experience and publications in leading journals, we offer tailored services for optimal results.
2. Rigorous Quality Management: Our mature quality systems adhere to ISO9001 standards, ensuring reliable reports.
3. Comprehensive Service: From probe design to bioinformatics analysis, we provide all-in-one consultation to delivery, with timely progress updates.
4. Advanced Equipment: Equipped with cutting-edge mass spectrometers like Thermo Fisher Orbitrap Exploris 480 and Bruker timsTOF, we support breakthrough research.

Our Service

Project	Lysine Post-Translational Modifications Based on High-Resolution Mass Spectrometry
Sample	Lysate, Live Cells, Tissue Samples
Hardware Platform	VanquishNeo UPLC coupled with Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific); EASY-nLC1200 UPLC coupled with Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific)
Project Duration	4-8 weeks
Deliverables	Project Report (Including gel images, site information, etc.)
Price	Click to consult