Absolute quantification analysis is a crucial technique in the field of proteomics, specifically designed to accurately determine the concentration or copy number of proteins or peptides in a sample. The core of this method lies in the precise calibration using known concentrations of the standards (usually isotopically labeled peptides). Absolute quantification analysis is of significant importance for both basic biological research and clinical applications. It not only provides more accurate protein expression information but also helps establish protein abundance databases, thereby deepening our understanding of protein function in physiological processes and pathological conditions.
Absolute quantification strategies include AQUA (Absolute Quantitation Criteria) and PRM (Parallel Reaction Monitoring), etc., which enable absolute quantification of target proteins.
1. Professional Excellence: Our team boasts extensive experience and publications in top journals, offering industry-leading technical services.
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3. Rigorous Quality Management: Adhering to ISO 9001 standards, our mature quality management system ensures the authenticity and reliability of our reports.
4. Systematic Project Management: From consultation to report delivery, we provide timely progress updates, ensuring customer satisfaction and efficient project execution.
5. Cutting-Edge Equipment: Equipped with advanced mass spectrometers like the Thermo Fisher Orbitrap Exploris 480 and Bruker timsTOF, we facilitate groundbreaking research.
Project | Peptide Relative/Absolute Quantification Analysis |
Sample | Tissue, Cell Precipitate, Lysate |
Hardware Platform | VanquishNeo UPLC liquid chromatography coupled with Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific); EASY-nLC1200 UPLC liquid chromatography coupled with Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific) |
Project Duration | 4-8 weeks |
Deliverables | Project Report (quantitative results of targeted peptides, quality control analysis, etc.) |
Price | Click to consult |