Products
Targeted Proteomics Analysis
Proteomics research can be divided into targeted and non-targeted proteomics based on whether the study focuses on specific types of proteins. Targeted proteomics is a research method that focuses on specific proteins or protein groups. It selectively detects and quantifies target proteins, providing highly sensitive and specific results, which are used to delve into protein functions and disease mechanisms. Compared to non-targeted proteomics, targeted proteomics demonstrates superior accuracy, sensitivity, and reproducibility, offering more precise and reliable tools for in-depth proteomics research.
Technical Platform
We provide targeted and untargeted proteomics analysis services based on mass spectrometry technology, aiming to offer comprehensive and in-depth support for protein research to our clients.
In the realm of targeted proteomics, ChomiX utilizes parallel reaction monitoring (PRM) as a core technology. PRM can sensitively detect low-abundance peptide fragments, effectively eliminate background protein interference, and achieve high-precision quantification, thereby efficiently validating and confirming the biologically differential proteins revealed by untargeted proteomics.
Compared to traditional targeted protein quantification methods like Western blot, targeted proteomics approaches do not require the development of antibodies and can simultaneously detect multiple proteins, with the option for absolute quantification when necessary.
Our Advantages
1. Professional Excellence: Our team boasts extensive experience and publications in top journals, offering industry-leading technical services.
2. Efficient Solutions: We employ reliable methods to drive projects forward swiftly, providing worry-free solutions.
3. Rigorous Quality Management: Adhering to ISO 9001 standards, our mature quality management system ensures the authenticity and reliability of our reports.
4. Systematic Project Management: From consultation to report delivery, we provide timely progress updates, ensuring customer satisfaction and efficient project execution.
5. Cutting-Edge Equipment: Equipped with advanced mass spectrometers like the Thermo Fisher Orbitrap Exploris 480 and Bruker timsTOF, we facilitate groundbreaking research.
Our Service
| Project | Targeted/Untargeted Quantitative Proteomics Analysis Set |
| Sample | Tissue, Cell pellets, Lysates |
| Hardware Platform | VanquishNeo UPLC coupled with Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) EASY-nLC1200 UPLC coupled with Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific) |
| Project Duration | 4 weeks |
| Deliverables | Project report (including qualitative/quantitative protein data tables, bioinformatics analysis, quality control analysis, etc.) |
| Price | Click to consult |
Case Study
To delve deeper into the binding sites of certain target proteins in cell samples treated with the drug compared to the control group, in order to reveal the molecular mechanisms related to the drug phenotype, we employed a targeted quantitative proteomics approach based on PRM (Parallel Reaction Monitoring) technology. Each group was processed with two biological replicates, allowing for precise identification and quantitative analysis of differential binding sites.
Selinexor (KPT330) treats multiple myeloma and diffuse large B-cell lymphoma by covalently binding to and inhibiting the activity of nuclear export protein 1 (XPO1_C528_LVVVGACGVGK). We treated HCT116 cells with different concentrations of KPT330 and found that the occupancy rate of the XPO1_C528 site by KPT330 could approach 100% at 1 μM. Additionally, the EC50 for intracellular site binding was determined to be 241 nM, which is comparable to the IC50 for the cellular inhibitory activity of KPT330 (150-500 nM), as indicated by the fitted curve.
